Table 2. The minimum and maximum allowable flux values for each reaction in the wild type, engineered cofactor imbalanced, and engineered cofactor balanced models were obtained using Flux Variability Analysis (FVA).

Reaction	Wild type model			Engineered cofactor imbalanced model			Engineered cofactor balanced model
	Min Flux*	Max Flux	Span	Min Flux	Max Flux	Span	Min Flux	Max Flux	Span
PGK	-11.40	-10.14	1.26	-16.21	-10.43	5.78	-16.00	-14.27	1.73
GAPD	10.14	11.40	1.26	10.43	16.21	5.78	14.27	16.00	1.73
PGM	-11.40	-10.07	1.33	-16.21	-10.34	5.86	-16.00	-14.17	1.83
ENO	10.07	11.40	1.33	10.35	16.21	5.86	14.17	16.00	1.83
GLYCDy	0.00	1.86	1.86	0.00	5.64	5.64	0.00	2.76	2.76
DHAK	0.00	1.86	1.86	0.00	5.64	5.64	0.00	2.76	2.76
G3PT	0.00	1.86	1.86	0.00	5.64	5.64	0.00	2.76	2.76
G3PD1ir	0.00	2.41	2.41	0.00	6.19	6.19	0.00	3.33	3.33
PYK	10.01	12.46	2.45	10.28	16.79	6.51	14.08	17.54	3.46
PYRDC	7.69	10.89	3.2	8.14	15.6	7.46	10.64	15.26	4.62

*mmol/gDCW/h.