Table 2. The minimum and maximum allowable flux values for each reaction in the wild type, engineered cofactor imbalanced, and engineered cofactor balanced models were obtained using Flux Variability Analysis (FVA).
Reaction | Wild type model | Engineered cofactor imbalanced model | Engineered cofactor balanced model | ||||||
Min Flux* | Max Flux | Span | Min Flux | Max Flux | Span | Min Flux | Max Flux | Span | |
PGK | -11.40 | -10.14 | 1.26 | -16.21 | -10.43 | 5.78 | -16.00 | -14.27 | 1.73 |
GAPD | 10.14 | 11.40 | 1.26 | 10.43 | 16.21 | 5.78 | 14.27 | 16.00 | 1.73 |
PGM | -11.40 | -10.07 | 1.33 | -16.21 | -10.34 | 5.86 | -16.00 | -14.17 | 1.83 |
ENO | 10.07 | 11.40 | 1.33 | 10.35 | 16.21 | 5.86 | 14.17 | 16.00 | 1.83 |
GLYCDy | 0.00 | 1.86 | 1.86 | 0.00 | 5.64 | 5.64 | 0.00 | 2.76 | 2.76 |
DHAK | 0.00 | 1.86 | 1.86 | 0.00 | 5.64 | 5.64 | 0.00 | 2.76 | 2.76 |
G3PT | 0.00 | 1.86 | 1.86 | 0.00 | 5.64 | 5.64 | 0.00 | 2.76 | 2.76 |
G3PD1ir | 0.00 | 2.41 | 2.41 | 0.00 | 6.19 | 6.19 | 0.00 | 3.33 | 3.33 |
PYK | 10.01 | 12.46 | 2.45 | 10.28 | 16.79 | 6.51 | 14.08 | 17.54 | 3.46 |
PYRDC | 7.69 | 10.89 | 3.2 | 8.14 | 15.6 | 7.46 | 10.64 | 15.26 | 4.62 |
*mmol/gDCW/h.