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. 2011 Nov 4;6(11):e27452. doi: 10.1371/journal.pone.0027452

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Ambruosi, et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Dose-response curve of the in vitro effects of DEHP on mitochondrial activity and intracellular ROS levels in single equine metaphase II stage oocytes expressed as Mitotracker Orange CMTM Ros (A) and DCF (B) fluorescence intensities. Oocytes treated with DEHP showed significantly higher ROS levels compared to controls. Values are expressed as arbitrary densitometric units (ADU). Pearson's correlation coefficents of Mitotracker Orange CMTM Ros and DCF fluorescent labelling in oocytes cultured in presence of DEHP (C). Numbers of analyzed oocytes per group are indicated on the top of each histogram. Student's t-Test: a, b P<0.05.