Table 1. B. anthracis putative cwss proteins and their anchoring sortase.

Protein name(s)	Ames numbera	cwss motif	predicted anchoring sortase	In vitro peptide cleavageb	In vivo anchoragec
BasA	BA4346	LPKTG	SrtA
Bas B	BA0871	LPATG	SrtA
Bas C	BA5258	LPATG	SrtA	[20]	NI-NTA chromatography after expression of BasC histidine fusion [20]
GamR/BasD	BA3367	LPKTG	SrtA		Gamma phage sensitivity [25]
BasE	BA3254	LPNAG	SrtA
BasH	BA0397	LPNTA	SrtC	[18]	Fluorescent microscopy after expression of fusion proteins [18], [47]
BasI	BA5070	LPNTA	SrtC
BasJ	BA0552	LGATG	SrtA
BasK/IsdC	BA4789	NPKTG	SrtB	[23]	Cell wall localization after fractionation of an IsdC histidine fusion [23]
BasL/IsdK, IsdX2	BA4787	NSKTA	SrtB		Cell associated localization after crude fractionation [26]
BasO	BA1088	LPHTG	NA

^aTIGR.org.

^b:2-aminobenzoyl-LPXTG motif-diaminoprpionic acid was incubated with purified sortase and cleavage is measured by fluorescence.

^c:in vivo anchoring of native, GamR, or GFP or histidine fusion proteins, BasC, BasH, BasI, IsdC, by different techniques.

NA; does not apply, no hydrophobic tail.