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. 1982 Sep 11;10(17):5239–5253. doi: 10.1093/nar/10.17.5239

Cobalt: an effector of E. coli recA protein activity.

J Resnick, R Sussman
PMCID: PMC320868  PMID: 6216455

Abstract

Studies of cation requirements in the recA-catalyzed proteolysis of lambda repressor and strand assimilation reactions have demonstrated that Co2+ significantly enhances both activities. In the presence of 4mM MgCl2, the optimal concentration of CoCl2 for proteolysis was 1mM. 2mM Co2+ increased the rate and extent of D-loop formation as measured by membrane filtration. Cobalt did not replace Mg2+ for the ssDNA-dependent ATPase activity of recA, and did not affect the rate of hydrolysis of ATP, measured over a wide range of DNA concentrations. Cobalt did prevent the Mg-dependent ssDNA renaturation catalyzed by recA protein. Membrane filter binding assays established that Co2+ increases the affinity of recA protein for ssDNA with ATP, dATP, or ATP gamma S as cofactors. The dissociation of recA protein from ssDNA-nucleoside triphosphate complex was much slower with CoCl2. This metal provides an excellent tool for dissecting the various activities inherent in recA protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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