Skip to main content
. Author manuscript; available in PMC: 2012 Dec 5.
Published in final edited form as: Virology. 2011 Oct 7;421(1):51–60. doi: 10.1016/j.virol.2011.08.008

Figure 3. Analysis of PKR colocalization with known cellular PKR inhibitors in WNV-infected cells.

Figure 3

Colocalization of PKR with known cellular PKR inhibitors. BHK cells were mock-infected or infected with WNV Eg101 (MOI of 5). At 30 h after infection, cells were permeabilized, fixed and blocked overnight. Cells were stained with anti-PKR and an antibody to a cellular PKR inhibitor and then with AlexaFluor488 (green) and AlexaFluor594/555 (red) conjugated secondary antibodies. PKR was detected with a mouse monoclonal anti-PKR antibody in the PP1a, p58ipk and Dcp1a experiments and with a rabbit polyclonal antibody in the Hsp70, Hsp90 and C114 experiments.