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. Author manuscript; available in PMC: 2012 Dec 5.
Published in final edited form as: Virology. 2011 Oct 7;421(1):51–60. doi: 10.1016/j.virol.2011.08.008

Figure 6. Analysis of active suppression of PKR activation in infected cells and of PKR anti-flaviviral activity.

Figure 6

(A) Poly(I:C)-mediated PKR autophosphorylation in WNV-infected cells. BHK cells were mock-infected or infected with WNV Eg101 (MOI of 5). At 20 or 30 h after infection, cells were transfected with 50 µg/ml of poly(I:C) in Celfectin II (+) or transfection reagent alone (−) for 1.5 h before cell lysis. PKR, phopho-Thr451 PKR and actin were detected in cell lysates by Western blotting after separation of proteins by 10% SDS-PAGE. (B) Viral yields produced by PKR−/− and wildtype MEFs infected with WNV Eg101 (MOI of 5). Samples of culture fluid were harvested at the indicated times, and infectivity titers were determined by plaque assays done in duplicate on BHK cells. Virus titers are expressed as log10 PFU/ml. Error bars indicate ± standard error of the mean (SEM) (n = 3).