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. Author manuscript; available in PMC: 2012 Nov 4.
Published in final edited form as: J Proteome Res. 2011 Oct 11;10(11):5175–5182. doi: 10.1021/pr200642c

Figure 4.

Figure 4

ARF enhances the interaction of VHL30 and PRMT3.

(A) U2OS cells were transfected with HA-VHL30, FLAG-PRMT3, and ARF, as indicated. Forty-eight hours after transfection, the interaction of HA-VHL30 with FLAG-PRMT3 was examined by anti-FLAG immunoprecipitation followed by anti-HA immunoblotting.

(B) U2OS cells were transfected with HA-ARF and FLAG-PRMT3 (left) or with HA-PRMT3 and FLAG-ARF (right) as indicated. Forty-eight hours after transfection, the interaction of ARF and PRMT3 was examined by anti-FLAG immunoprecipitation followed by anti-HA immunoblotting.