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. Author manuscript; available in PMC: 2012 Nov 4.
Published in final edited form as: J Proteome Res. 2011 Oct 11;10(11):5175–5182. doi: 10.1021/pr200642c

Figure 5.

Figure 5

VHL30 and ARF recruit PRMT3 to p53.

(A) VHL30 binds p53.

Left, Cells were transfected with HA-P53, FLAG-VGL30, and ARF, as indicated. The interaction of HA-P53 with FLAG-VHL30 was examined by anti-FLAG immunoprecipitation followed by anti-HA immunoblotting.

Right, Cells were transfected with FLAG-VHL30 with or without ARF, as indicated. The interaction of endogenous p53 and FLAG-VHL30 was examined by anti-FLAG immunoprecipitation followed by anti-HA immunoblotting.

(B) VHL30 and ARF recruit PRMT3 to p53.

Cells were transfected with FLAG-PRMT3, VHL30, and ARF as indicated. The interaction of FLAG-PRMT3 and endogenous p53 was examined by anti-FLAG immunoprecipitation followed by anti-p53 immunoblotting.