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VHL30, ARF, and PRMT3 induce asymmetric arginine di-methylation of p53.
Cells were transfected with FLAG-p53, VHL30, ARF, and PRMT3, as indicated. Arginine methylation of FLAG-p53 was examined by anti-FLAG immunoprecipitation under denaturing conditions followed by anti-asymmetric dimethyl arginine or anti-symmetric dimethyl arginine immunoblotting.
