Fig. 1.

OFQ/N increases NFκB p65 and p50 nuclear accumulation over time. SH-SY5Y cells were treated with 1 µM OFQ/N for the indicated time. Nuclear fractions were extracted and protein levels determined by immunoblotting with antibodies specific for NFκB p65, p50, or nucleolin. NFκB immunoreactivity was normalized to nucleolin (loading control) and expressed as fold over basal. Basal values were 0.9±0.4 for p50 and 0.4±0.2 for p65. OFQ/N increases nuclear accumulation of p65 and p50 by 2 h (1.9- and 2.2-fold, respectively; *p<0.05, **p<0.01, compared to basal). Representative blot and mean densitometric analysis ± SEM of three to five experiments are shown