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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer Prev Res (Phila). 2011 Oct 25;4(11):1743–1751. doi: 10.1158/1940-6207.CAPR-11-0365

Figure 6. Nicotine only activates components of PI3K and MAPK under serum starvation conditions.

Figure 6

Immunoblotting analysis of Cl25 cells after one-hour nicotine treatment at indicated doses in 0.5% serum (A) or 5% serum (B). C, SRB assay of CL25 cells in 5% serum. Prior to nicotine treatment, OD570 values were approximately 0.1. The first bar for each time point is untreated cells. Nicotine doses (from left to right for each time point) were 1 nM, 10 nM, 100 nM, 1 µM and 10 µM. Any differences are not significant.