Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Biomech Model Mechanobiol. 2011 Jan 21;10(6):939–953. doi: 10.1007/s10237-010-0285-8

Table 1.

Applied stimuli for Mechanical Panel experiments to determine the dose–response of MSCs to different magnitudes and frequencies of mechanical stimulation

Stimulus Neonatal/sub-physiologic stimulus Hypotensive stimulus Normotensive stimulus Hypertensive stimulus
Cyclic stretch 1%, 2.75 Hz (CS-1HF) 1%, 1 Hz (CS-1) 5%, 1 Hz (CS-5) 10%, 1 Hz (CS-10)
Cyclic pressure 90/70mmHg, 2.75 Hz
    (CP-90HF)		 90/70mmHg, 1 Hz
    (CP-90)		 120/80mmHg, 1 Hz
    (CP-120)		 180/140 mmHg, 1 Hz
    (CP-180)
Shear stress 1 dyne/cm2 (LSS-1) 5 dynes/cm2 (LSS-5) 10 dynes/cm2 (LSS-10) 20 dynes/cm2 (LSS-20)
Control Static incubator conditions (37°C, 5% CO2, 95% humidity)

Neonatal frequency and blood pressure values were determined form normal newborn heart rate and blood pressure values found in Whaley and Wong 1999. Stretch and shear magnitudes were derived from an excellent review by Oluwole et al. 1997. Parenthetical terms denote abbreviations for each of the stimuli. For example, CS-1 denotes 1% 1 Hz cyclic stretch, and LSS-1 denotes 1 dyne/cm2 laminar shear stress. CS-1 HF denotes cyclic stretch at 1% at 2.75 Hz stimulation (high frequency)