Skip to main content
. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Biomaterials. 2011 Oct 7;33(1):128–136. doi: 10.1016/j.biomaterials.2011.09.058

Fig 4. Gene expression and Ca deposition analyses of hMSCs cultured on topographic surfaces and planar control.

Fig 4

Fig 4

Fig 4

Expression of two osteogenic markers, RUNX2 (A) and BGLAP (B), was measured by quantitative PCR at day 3, 7, 10 and 14. The expression of Runx2 or BGLAP was first normalized to the expression of endogenous control TBP (TATA-binding protein), and then normalized to the Runx2 or BGLAP expression of the planar control at each day. The hMSCs on 400 nm pitch expressed significantly higher RUNX2 than planar surfaces at day 3 and 14 (A). hMSCs on patterned surfaces expressed consistently higher BGLAP than cells on planar surfaces (B). Normalized calcium deposition of hMSCs on topographic surfaces and planar control at day 7, 14 and 21 (C). The total calcium depositions were measured and normalized to the cell number on each surface. hMSCs on 400 nm pitch showed significantly higher normalized calcium deposition compared to planar or 4000 nm pitch surfaces at day 7 and 14. All data are presented as the mean ± SEM.