Figure 7. Comparison of parameters of mitochondrial function between Day 0 undifferentiated SH-SY5Y cells and Day 5 differentiated cells in response to HNE treatment.
(A) Basal OCR after HNE (determined as OCR at 50 min after HNE injection normalized against the OCR before HNE injection) was plotted with increasing doses of treatment with HNE. (B) Maximal OCR (determined as OCR after FCCP injection normalized against OCR before oligomycin injection) was plotted with increasing doses of treatment with HNE. (C) Reserve capacity (determined as OCR after FCCP injection minus OCR before oligomycin injection followed by normalization against OCR before oligomycin injection) was plotted with increasing doses of treatment with HNE. Data = mean ± SEM. *p <0.05 Student t-test compared to Day 0. # p <0.05 compared to 0 µM treatment.