Figure 2.
Oleosin mRNA accumulation in MDEs after treatment with 10 μm ABA and 30 μm JA for various times. Relative intensities of densitometry scans of autoradiographs from slot blots of total RNA (5 μg/slot) probed with a [32P]dCTP-labeled oleosin cDNA clone are plotted. Values are the mean of three experiments ± se. The same blot was reprobed with pGS43 (Harada et al., 1989), a gene expressed constitutively in developing zygotic embroys. The signal obtained from this hybridization was used to correct for slight variations in RNA loading.