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. Author manuscript; available in PMC: 2012 Nov 4.
Published in final edited form as: J Proteome Res. 2011 Oct 11;10(11):5191–5198. doi: 10.1021/pr200662b

Figure 2. SAFB2 ubiquitination by BRCA1/BARD1.

Figure 2

293 T cells were transfected with His-SAFB2, HA-ubiquitin, BRCA1, and BARD1 as indicated. Forty-eight hours after transfection, His-SAFB2 was isolated using nickel affinity chromatography under denaturing conditions. Ubiquitination of His-SAFB2 was analyzed by anti-HA immunoblotting (top), and His-SAFB2 was detected by anti-SAFB immunoblotting (bottom).