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. Author manuscript; available in PMC: 2012 Oct 28.
Published in final edited form as: Circ Res. 2011 Sep 15;109(10):1141–1152. doi: 10.1161/CIRCRESAHA.111.246363

Figure 2. J774 macrophage triglyceride and sterol contents are increased by hypoxia under normoglycemic conditions.

Figure 2

J774 macrophages were incubated for 24 h under normoxic or hypoxic (1% O2) conditions in either normal or hyperglycemic medium containing 15% FBS (panels A-C) or serum free (panel D). Lipid assays were performed and shown are the effects of hypoxia on cellular levels of: A, Triglycerides; B, Total sterol (desmosterol+cholesterol); C, Total and free sterol in non-cholesterol-loaded cells. Note that the assays for total and free sterol are independently performed, so that minor assay variations may appear as small differences, such as those in the hypoxic cells; D, Same as panel C, but in cholesterol-loaded cells. The majority of total sterol is in the free form (~86-87%) in both conditions, despite the massive increase in sterol content in hypoxia. All panels: These experiments were performed 3 times in triplicate. Statistical significance is * for p<0.05, ** for p<0.01, and *** p<0.001. NS- non significant.