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. Author manuscript; available in PMC: 2012 Oct 28.
Published in final edited form as: Circ Res. 2011 Sep 15;109(10):1141–1152. doi: 10.1161/CIRCRESAHA.111.246363

Figure 7. Hypoxic regions of atherosclerotic plaques show a gene expression pattern similar to those in macrophages incubated under hypoxic conditions.

Figure 7

ApoE-/- mice were fed the Western diet for 16 weeks and aortic root sections were prepared for laser capture microdissection. Samples were isolated from normoxic and hypoxic regions (positive for both VEGF and GLUT-1 staining; on-line Methods). J774 macrophages and BMDM were exposed to hypoxia in vitro for 24 h. RNA was isolated from laser-captured samples and cultured macrophages, and mRNA abundances was measured using qRT-PCR with resulted normalized to Cyclophilin A. Note that the graphs for ABCA-1 and HMGCoA reductase are repeated from Figures 4B and 3B, respectively. Statistical significance is indicated by * for p<0.05 and ** for p<0.01. The in vitro experiments were performed 3 times in triplicate and the LCM samples were isolated from 6 animals.