Table 4.
Genotypic and phenotypic characterization of oil-degrading bacteria isolated from beach sands impacted by the DH oil spilla
| Closest BLAST match | % Similarity | GenBank accession no. | Enrichment condition | Quantitative test for oil degradation |
|---|---|---|---|---|
| Pseudomonas pachastrellae | 100 | EU603457 | Anoxic | |
| Pseudidiomarina maritima | 99.9 | EU600203 | Anoxic | Yes |
| Marinobacter hydrocarbonoclasticus | 99.6 | DQ768638 | Anoxic | |
| Shewanella algae | 99.2 | GQ372877 | Anoxic | |
| Vibrio alginolyticus | 97.7 | GQ455008 | Anoxic | |
| Pseudomonas stutzeri | 99.7 | GU396288 | Anoxic | Yes |
| Alcanivorax dieselolei | 100 | AB453732 | Anoxic | |
| Vibrio hepatarius | 98.6 | EU834019 | Anoxic | Yes |
| Marinobacter vinifirmus | 99.2 | FJ161339 | Anoxic | |
| Marinobacter vinifirmus | 99.1 | FJ161339 | Anoxic | |
| Vibrio sp. | 99.9 | HM640395 | Oxic | |
| Acinetobacter sp. | 100 | FJ876296 | Oxic | Yes |
| Pseudoalteromonas sp. | 99.8 | AY394863 | Oxic | |
| Acinetobacter venetianus | 99.8 | DQ912805 | Oxic | |
| Bacillus sp. | 99.2 | HQ588864 | Anoxic | |
| Halomonas shengliensis | 99.7 | EF121853 | Anoxic | |
| Vibrio hepatarius | 99 | HM584097 | Anoxic | |
| Vibrio alginolyticus | 99.3 | GQ455008 | Anoxic | |
| Marinobacter hydrocarbonoclasticus | 100 | DQ768638 | Anoxic | Yes |
| Labrenzia sp. | 99.3 | EU440961 | Oxic MPN | |
| Alcanivorax sp. | 99.9 | AB435642 | Oxic MPN | |
| Microbulbifer sp. | 98.7 | GQ334398 | Oxic MPN | |
| Microbacterium schleiferi | 99.9 | EU440992 | Oxic MPN | |
| Marinobacter hydrocarbonoclasticus | 99.4 | DQ768638 | Oxic MPN |
a
The results are based on comparison of SSU rRNA gene sequences of the isolates to the sequence that shows the highest sequence identity to the isolate. All strains were isolated in a minimal artificial seawater medium with DH source oil as the sole carbon and electron source. The oil degradation activity was quantified for representative isolates using the gravimetric assay described in Materials and Methods. Anoxic enrichment conditions indicate denitrifying enrichments.