Fig. 5.

Unrooted maximum likelihood phylogram of Accumulibacter-related 16S rRNA gene sequences. Bootstrap values of >50% and generated from 1,000 bootstrap resamplings are shown next to nodes. Roman numerals refer to Accumulibacter clades as designated previously (21). Sequences obtained in this study are shaded. The clone names are read as follows: the number following d refers to the reactor's operation day of sampling; AG_Prop refers to the propionate- and AG_Acet to the acetate-cultivated aerobic granules. The numbers in parentheses refer to the frequency of that sequence with respect to the total sequences in the respective clone library. The scale bar indicates changes per site. The phylogram was constructed using version 5 of the MEGA software (53).