Fig. 2.

Expression of MmFAMT leads to formation of FAMEs and 3-OH-FAMEs. E. coli BL21 ΔmetJ::kan cells were transformed with double-expression vectors expressing Mmar_3356 (A and B) or Msmeg_4347 (C) and rat MAT. An FFA (A) or 3-OH-FFA (B and C) cocktail mixture was added exogenously to each culture during induction. Lipids from cell pellets and supernatants were extracted 20 h after induction, and FAMEs and 3-OH-FAMEs were quantified as described in Materials and Methods. Data are representative of experiments done three independent times.