Fig. 7.

Expression of phaG and tesB with FAMT leads to production of FAME and 3-OH-FAME. E. coli BL21 ΔmetJ::kan cells were transformed with Duet coexpression vectors expressing PhaG, TesB, FAMT, and rMAT. (A and B) Cells were collected 24 h and 48 h after induction, and FAMEs (A) and 3-OH-FAMEs (B) in the cell pellet and supernatant were quantified as described in Materials and Methods. (C) Growth curves of uninduced (triangles) and induced (filled squares) E. coli BL21 ΔmetJ::kan transformed plasmids encoding PhaG, TesB, FAMT, and rMAT. Aliquots from each culture were collected at 4, 8, 20, 30, and 48 h postinduction and analyzed for growth and methyl ester production. For the induced culture, the numbers below each time point represent methyl ester production in μM. ND, not detectable. Error bars represent the standard deviations from triplicate experiments.