Fig. 5.

Titers of 16- to 18-carbon-chain-length fatty acids (white), which are primarily associated with membrane lipids, and 8- to 14-carbon-chain-length fatty acids (gray), which are primarily either FFAs or minorly associated with lipid A of (a) strain RL13 (ΔpspF) expressing BTE-H204A (BTE-) or BTE (BTE+), and (b) strains RL09 (marA::kan), RL10 (rob::kan), and RL11 (soxS::kan) expressing BTE-H204A or BTE. Strain RL08 was used as a negative control against gene deletion strains. All fatty acid samples were taken after 24 h of shake flask growth in LB medium supplemented with 0.4% glycerol as described in Materials and Methods.