Table 3.
Changes in expression of selected genes in BTE-expressing strains versus BTE-H204A expressing strains as measured by qPCR
| Locus | Gene name | Fold changea (mean ± SE) at phase |
||
|---|---|---|---|---|
| Mid-log | Early stationary | Mid-stationary | ||
| b0954 | fabA | −1.12 ± 0.58 (−1.28, 0.087) | −4.05 ± 1.43 (−2.54, 0.004) | −8.15 ± 2.92 (−4.80, 0.000) |
| b1307 | pspD | 1.14 ± 0.62 (−1.03, 0.82) | 38.8 ± 11.9 (4.99, 0.002) | 57.0 ± 24.5 (9.60, 0.002) |
| b2237 | inaA | 1.08 ± 0.71 (1.58, 0.001) | 1.80 ± 0.61 (7.72, 0.000) | 2.76 ± 0.89 (5.72, 0.000) |
a
Sampling times were as defined in Materials and Methods. Standard errors are provided between biological triplicates. Values in parentheses below each qPCR fold change value correspond to the measured fold change observed in the equivalent DNA microarray experiment, with the P value provided in italics.