Table 5.
Protein changes for BTE-expressing strains versus BTE-H204A-expressing strains cultured in EZ rich defined medium supplemented with 0.2% glucose in shake flasks (EZ glucose) or in EZ rich defined medium supplemented with 0.4% glycerol (EZ glycerol)a
Locus | Protein | Fold change between BTE-expressing strains vs BTE-H204A-expressing strains |
|||||
---|---|---|---|---|---|---|---|
EZ glucose, BTE+ ACC− |
EZ glycerol |
||||||
BTE+ ACC− |
BTE+ ACC+ |
||||||
Early stationary | Mid-stationary | Late log | Mid-stationary | Late log | Mid-stationary | ||
PutA | 2.10 | 2.20 | −4.32 | ||||
b1304 | PspA | 5.52 | 2.28 | ||||
b1493 | GadB | 4.63 | |||||
b1611 | FumC | 3.53 | |||||
b1743 | Spy | 5.24 | |||||
b2323 | FabB | −4.20 | −3.46 | −2.36 | −5.85 | −2.46 | −7.00 |
b3506 | Slp | 2.10 | −2.11 |
Cultures were grown in fermentors and sampled at the times specified in the text. P values for all fold changes shown were less than 0.05. No significant changes in expression levels were measured at the mid-log phase sampling point for EZ glucose cultures.