Timeline and frequency of bacterium-induced internal egg hatching. (A) Cumulative percentage of worm bags appearing over the course of the C. elegans reproductive period. Data are compiled, with all bacterial strains included, from all experiments of worm bag frequency determination (n = 55). Means ± standard deviations (SDs) are graphed. (B) Frequency of worm bags in C. elegans populations exposed to E. coli strains OP50 (n = 381), ED1A (n = 291), IAI13 (n = 245), MG1655 (n = 582), IAI1 (n = 378), F11 (n = 235), and 536 (n = 905) and to E. faecalis strain OG1RF (n = 313). Data are compiled from at least three independent experiments. Bagging frequencies were analyzed using a one-way analysis of variance (ANOVA), followed by Dunnett's multiple-comparison test against OP50. Means ± standard errors of the mean (SEM) are graphed. ns, not significant; *, P < 0.05; ***, P < 0.0001.