Fig. 5.

Effect of hemin and holotransferrin on the expression of RNA III. S. aureus MW2 cells cultured in CS and CDM(−) with or without 3 μM FeCl₃, hemin, or holotransferrin were collected at the exponential phase. The expression of RNA III was determined by quantitative PCR by the method described in Materials and Methods. The data shown represent means and SD of measurements performed in triplicate. The asterisks indicate significant decreases compared with the medium with no addition of iron sources as determined by Dunnett's test: ***, P < 0.001.