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. 2011 Nov;77(22):8097–8105. doi: 10.1128/AEM.05316-11

Fig. 9.

Fig. 9.

Effect of serum albumin on the expression of RNA III. (A) Serum albumin from calf serum was purified using ToyoPearl AF-Blue HC-650 and then added to CDM(−). Small portions of CS and CDM(−) containing purified albumin were subjected to SDS-PAGE using a 7.5% polyacrylamide gel and then stained with Coomassie brilliant blue. M, size marker; lane 1, CS (3 μg); lane 2, CDM(−) containing purified albumin from CS (2 μg). (B) S. aureus MW2 cells cultured in CS, CDM(−), and CDM(−) containing purified serum albumin from CS, with or without 3 μM iron sources, were collected at the exponential phase. The expression of RNA III was determined by quantitative PCR by the method described in Materials and Methods. The data shown represent means and SD of measurements performed in triplicate. The asterisks indicate significant decreases compared with no addition of an iron source as determined by Dunnett's test: ***, P < 0.001.