Table 2.

General features of the pAKD plasmids

Plasmid	Soil of origina	Isolation methodb	Size (bp)	IncP-1 subgroup	Phenotypec
pAKD1	StendS	BM	58,246	β	Hgr Spr Smr Sur
pAKD4d	StendS	BM	56,803	δ	Hgr
pAKD14	Ås	BM	48,624	β	Hgr
pAKD15	Ås	BM	66,550	β	Hgr
pAKD16	Ås	TM	74,971	ε	Hgr
pAKD17	Ås	BM	51,755	β	Hgr
pAKD18	Ås	BM	67,298	β	Hgr
pAKD25	Garpestad	BM	75,067	ε	Hgr
pAKD26	Garpestad	BM	82,401	β	Hgr
pAKD29	StendS	BM	55,020	β	Hgr
pAKD31	Ås	BM	54,140	β	Hgr
pAKD33	Ås	BM	57,974	β	Hgr
pAKD34	Ås	BM	86,438	ε	Hgr

^aSoil of origin—agricultural fields growing cereal and vegetables in StendS and Garpestad and only cereal in Ås.

^bIsolation method: BM, biparental mating; TM, triparental mating (8, 9).

^cPhenotype: phenotype confirmed. Hg^r, mercury resistance; Sm^r, streptomycin resistance; Sp^r, spectinomycin resistance; Su^r, sulfonamide resistance.

^dPreviously published (27).