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. 2011 Nov;77(22):7915–7923. doi: 10.1128/AEM.05274-11

Fig. 3.

Fig. 3.

(a) Survival of environmental isolates in acidic conditions. LB medium (pH 2.5) was inoculated with ∼105 cells and incubated at 37°C with shaking for 2 h. Percent survival was calculated as (final CFU/ml)/(initial CFU/ml) × 100, and values of <0.0001% are indicated with an asterisk. ECA01Suc++ is a succinate-selected RpoS mutant from ECA01, and AZB07 is an RpoS mutant isolated from beach sand. The experiment was done in triplicate, and values represent means ± standard errors of the means. (b) Survival of environmental isolates in oxidative stress. LB medium with 15 mM H2O2 was inoculated with ∼107 cells, and the percent survival was calculated over time. At 40 min, ECA01Suc++ and AZB07 had <0.0001% survival. ECA01Suc++ is a succinate-selected RpoS mutant from ECA01, and AZB07 is an RpoS mutant isolated from beach sand. Experiments were done in triplicate, and values represent means ± SEM.