Table 3.
Identified mutations in the rpoS gene that predict a nonfunctional RpoS protein in environmental E. coli isolates
| Isolate | Source type | Type of mutation | Effect of mutation on amino acid sequence | Location (nucleotides) | rpoS accession no. |
|---|---|---|---|---|---|
| ECE12 | Untreated combined sewer overflow | 5-bp deletion | Frameshift, short protein | 255–259 | JN191271 |
| BNB03a | Beach sand | 1-bp deletion | Frameshift, short protein | 378 | JN191252 |
| AZB07 | Beach sand | G→A transition | TGG→TAG (amber stop codon) | 443 | JN191250 |
| ASC02 | Sewage treatment plant final effluent | 1,329-bp insertion (IS10 transposase) and 9-bp duplication | Short protein | 776 | JN191249 |