Table 1.
DNA yields and fragmentation results after completion of the Nextera protocol
| Phage | Typea | Final DNA concn (ng/μl) | Total DNA (ng)b | Concn fragments of 300–800 bpc (ng/μl) | % total DNA (300–800 bp)d | Fragment size of greatest abundancec (bp) | Avg read length for 454 sequences (bp)e |
|---|---|---|---|---|---|---|---|
| Angelica | M | 12 | 307 | 9 | 79 | 569 | 373 |
| Athena | M | 33 | 858 | 27 | 81 | 1,402 | 382 |
| Avrafan | M | 21 | 322 | 11 | 49 | 843 | 355 |
| Blue7 | M | 13 | 332 | 9 | 68 | 398 | 336 |
| Wee | M | 19 | 482 | 12 | 65 | 529 | 348 |
| TUSD 1 | Cy | 34 | 915 | 20 | 58 | 1,392 | 320 |
| TUSD 14 | Cy | 21 | 571 | 11 | 51 | 1,348 | 307 |
| TUSD 20 | Cy | 20 | 536 | 10 | 52 | 732 | 317 |
| TUSD 21 | Cy | 29 | 778 | 11 | 39 | 1,359 | 315 |
| Mock metagenome | 22 | 708 | 12 | 55 | 1,151 | 349 |
a
M, mycobacteriophage; Cy, cyanophage.
b
x ± SD = 581 ± 226 ng.
c
Data were determined using an Agilent Bioanalyzer.
d
x = 60%.
e
The average read lengths were calculated after removal of sequences of less than 100 bp.