Table 3.

Predicted versus experimental coverage and percent abundance of reads for each phage in the mock metagenome

Phage	Virus typea	Genome size (bp)	GC content (%)	Predicted result			Experimental result
							Recruitment to reference sequence			Mock assembly
				No. of reads	Coverage	Abundance of reads (%)	No. of reads	Coverageb (± SD)	Abundance of reads (%)	No. of contigs	Longest contig (bp)	N50 contig (bp)
T4	C	166,000	35	20,000	47	28.1	31,049	61.6 (14.6)	48.2	3	169,170	169,170
Catera	M	153,766	65	14,000	36	19.7	7,592	18.0 (8.6)	11.8	4	85,298	85,298
Fruitloop	M	58,471	62	12,000	82	16.8	8,745	53.3 (18.9)	13.6	1	58,308	58,308
Gumball	M	64,807	60	10,000	62	14.0	9,999	56.4 (18.8)	15.5	1	64,807	64,807
Omega	M	110,865	61	8,000	29	11.2	3,362	10.9 (5.2)	5.2	6	41,626	20,153
Porky	M	76,312	63	4,000	21	5.6	2,038	9.7 (5.8)	3.2	7	34,434	17,944
Solon	M	49,487	64	2,000	16	2.8	715	5.2 (3.4)	1.1	12	10,570	7,363
P-SS2	Cy	107,530	52	1,000	3.7	1.4	757	2.5 (2.2)	1.2	81	2,924	1,313
S-SM1	Cy	174,079	41	200	0.5	0.3	205	0.4 (0.9)	0.3	50	915	463
Total				71,200				64,718 (257 unassigned, 64,462 assigned)		172 (6 unassigned, 166 assigned)

^aC, coliphage; M, mycobacteriophage; Cy, cyanophage.

^bCoverage is defined as number of bases per position in the consensus sequence.