Table 1.
Bacterial strains and recombinant plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| H10407 | Wild-type ETEC strain; serotype O78:H11; CFA/I LT+ STh+ STp+ | 9 |
| jf1412 | fliC mutant of H10407; nonmotile; Kmr | 30 |
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL StrrendA1 nupG | Invitrogen |
| jf876 | ΔlacZYA derivative of H10407 | 8 |
| BL21(DE3) | F−ompT hsdSB(rB− mB−)dcm gal (DE3) | |
| Plasmids | ||
| pJL017 | etpBA cloned into pBAD/Myc-HisA, with etpA in frame with Myc and His6 coding regions | 28 |
| pJL030 | etpC gene cloned into pACYC184; Cmr | 30 |
| pGPM1034 | cexE gene cloned into pET33b in frame with His6 coding region | 24 |