Table 1.
Specificity and sensitivity of the MAb-based serotyping assay
| MAb no. | MAb designation | Serotype specificitya | IC50 (ng/ml)b |
|---|---|---|---|
| 1 | Hyp1G4 | 1 | 8 |
| 2 | Hyp2M2 | 2 | 10 |
| 3 | Hyp3M6 | 3 | 6 |
| 4 | Hyp4M3 | 4 | 6 |
| 5 | Hyp5M3 | 5 | 12 |
| 6 | Hyp6AM3 | 6A | 3 |
| 7 | Hyp6BM1 | 6B | 77 |
| 8 | Hyp6DM5 | 6C/6D | 14 |
| 9 | Hyp7FM15 | 7F/7A | 52 |
| 10 | Hyp8M2 | 8 | 10 |
| 11 | Hyp9NM3 | 9N | 8 |
| 12 | Hyp9VM7 | 9V/9A | 1 |
| 13 | Hyp10AG3 | 10A/39 | 58 |
| 14 | Hyp11AM2 | 11A/11D/11F | 13 |
| 15 | Hyp11AM1 | 11E/(11A/11D/11F) | 15 |
| 16 | Hyp12FM3 | 12F | 26 |
| 17 | Hyp14M11 | 14 | 5 |
| 18 | Hyp15BG5 | 15B/(15C) | 68 |
| 19 | Hyp17FM6 | 17F/17A | 6 |
| 20 | Hyp18CM1 | 18C | 5 |
| 21 | Hyp19AG1 | 19A | 5 |
| 22 | Hyp19FM3 | 19F | 5 |
| 23 | Hyp20G5 | 20 | 29 |
| 24 | Hyp22FM2 | 22F/22A | 4 |
| 25 | Hyp23FG3 | 23F | 16 |
| 26 | Hyp33FG1 | 33F/33A | 18 |
a
Serotypes in parentheses are those with partial cross-reaction. Hyp10AG3 does not cross-react with 10B, unlike Danish factor serum 10d (12).
b
The PS concentration required to inhibit 50% of MAb binding to the enzyme-linked immunosorbent assay plate.