Fig. 3.

N. gonorrhoeae-specific bactericidal activity of sera from immunized mice detected using an antibody complement-mediated bactericidal activity assay. A series of diluted serum samples were mixed with gonococcal cells and incubated. Normal human fresh serum was added into the mixtures as complement sources. Then, the mixtures were plated onto GC chocolate agar and cultured. The recovered gonococcal colonies from the mixtures containing the immunized serum samples were enumerated and compared to those from negative controls (gonococci incubated with normal mouse serum). For all groups, n = 3.