Fig. 4.
Respiratory burst effect enhanced by immune sera. Luminol-enhanced chemiluminescence was measured using a 1420 Victor D Multilabel Counter (Wallac) at approximately 2-min intervals in 96-well white Nunc microtiter plates containing a mixture of pooled serum samples, 2 × 106 gonococcus cells, 10% normal human serum, 2 × 105 Percoll-isolated human PMNs, and 0.1 μM luminol. The positive control contained zymosan A but not mouse serum or bacterial cells; the negative control was gonococcus cells incubated in normal mouse serum. Representative data from one of the three experiments are shown.