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. 2011 Nov;18(11):1889–1894. doi: 10.1128/CVI.05225-11

Fig. 2.

Fig. 2.

Procedure for pPNT. (A) Schematic representation of the pPNT procedure. O/N, overnight. (B) Interpretation of pPNT. (Top) Neutralization curve of PV pseudoviruses with a human serum sample. The threshold levels for determination of the neutralizing-antibody titer are shown by the dotted lines (≤20%, 2.5%, and 10% for type 1, 2, and 3 PV, respectively), with neutralizing-antibody titers circled (64, 32, and 128 for type 1, 2, and 3 PV, respectively). (Bottom) Original data for the neutralization curve shown at the top. The wells that showed the highest dilution below the threshold values in two lines of measurement are circled. The wells that showed the highest dilutions below the threshold values in the measurement are indicated by arrows for each type of PV pseudovirus.