Table 1.
Vaccination groups and administration strategy for HBV DNA vaccines
| Group | Vaccine or reagent | Dose | Route |
|---|---|---|---|
| A1 | PBS | 50 μl | i.m. |
| A2 | pVRC-SS1 | 50 μg | i.m. |
| A3 | pVRC-CS1 | 50 μg | i.m. |
| B1 | PBS | 50 μl | i.m.-EPa |
| B2 | pVRC-SS1 | 50 μg | i.m.-EP |
| B3 | pVRC-CS1 | 50 μg | i.m.-EP |
| C1 | PBS | 50 μl | i.d.-EPb |
| C2 | pVRC-SS1 | 10 μg | i.d.-EP |
| C3 | pVRC-CS1 | 10 μg | i.d.-EP |
| C4 | pVRC-SS1 + pVRC-CS1 | 10 μg + 10 μg | i.d.-EP |
a
EP parameters: i.m. (pulse generator [ECM830; BTX] with a two-array needle electrode), 27.5 v/mm for 20 ms, 8 times, with a 1-s interval between the EPs.
b
EP parameters: i.d. (square-wave pulse generator [ECM830; BTX] with a caliper electrode consisting of two brass plate electrodes, 1 by 1 cm), 10 v/mm for 15 ms, 3 times, followed by reverse electroporation at 7.5 v/mm for 20 ms, 3 times, with a 1-s interval between the EPs.