Fig. 1.

Features of evaluated TSSA molecules. (A) Schematic illustration of TSSA products showing the predicted signal peptide (SP) and GPI-anchoring signals. The sequences of the TSSA I and TSSA VI regions expressed as GST fusion proteins are indicated. Variable positions between both proteins are shaded. The predicted SP cleavage site is indicated with an inverted triangle, and amino acids (aa) predicted to be O-glycosylated in the TSSA VI protein are indicated with asterisks. B-cell epitopes recognized by human Chagasic sera in the TSSA VI protein are underlined. (B) The purity of the recombinant GST fusion proteins used for the CL-ELISAs was ascertained by silver staining of an SDS-PAGE gel containing 1 μg of the indicated protein. Molecular size markers (in kDa) are indicated.