Table 1.
Proteins with altered expression in eosinophils from a patient with hypereosinophilia from fascioliasis
| Protein name | SwissProt accession no. | Fold changea | Theoretical/observed |
Peptide count | Expectation valueb | Function | Pick no.c | |
|---|---|---|---|---|---|---|---|---|
| pI | Mr | |||||||
| 14-3-3 protein zeta/delta | P63104 | 2.1 | 4.73/5.17 | 27.9/21.31 | 12 | 1.58E-29 | Cell signaling | 10 |
| 60S ribosomal protein L4 | P36578 | 5.6 | 11.07/7.6 | 47.95/73.91 | 12 | 0.16 | Protein synthesis | 42 |
| Actin, cytoplasmic 2 | P63261 | 4.9 | 5.31/4.91 | 42.11/11.06 | 4 | 1.26E-08 | Actin-related protein | 22 |
| Actin, cytoplasmic 2 | P63261 | 5.1 | 5.31/6.23 | 42.11/19.43 | 6 | 7.92E-16 | Actin-related protein | 17 |
| Actin, cytoplasmic 2 | P63261 | 2.8 | 5.31/5.7 | 42.11/20.2 | 9 | 3.97E-22 | Actin-related protein | 13 |
| Actin, cytoplasmic 2 | P63261 | 2.2 | 5.31/5.51 | 42.11/21.43 | 13 | 3.97E-42 | Actin-related protein | 12 |
| Actin, cytoplasmic 2 | P63261 | 2.5 | 5.31/5.35 | 42.11/21.89 | 12 | 1.26E-37 | Actin-related protein | 11 |
| Actin, cytoplasmic 2 | P63261 | 2.9 | 5.31/5.34 | 42.11/24.02 | 8 | 2.51E-29 | Actin-related protein | 9 |
| Actin, cytoplasmic 2 | P63261 | 4.0 | 5.31/5.82 | 42.11/24.93 | 10 | 6.29E-23 | Actin-related protein | 14 |
| Actin, cytoplasmic 2 | P63261 | 4.2 | 5.31/5.15 | 42.11/25 | 9 | 6.29E-28 | Actin-related protein | 8 |
| Actin, cytoplasmic 2 | P63261 | 2.0 | 5.31/5.1 | 42.11/25.09 | 12 | 1.58E-42 | Actin-related protein | 6 |
| Actin-like protein 3 | P61158 | 4.4 | 5.61/7.33 | 47.8/25.03 | 16 | 3.97E-25 | Actin-related protein | 35 |
| Actin-like protein 3 | P61158 | 3.1 | 5.61/7.46 | 47.8/25.07 | 15 | 5.00E-34 | Actin-related protein | 36 |
| Alpha-enolase | P06733 | 2.7 | 7.01/6.58 | 47.48/24.16 | 15 | 9.98E-22 | Carbohydrate metabolism | 44 |
| Annexin A1 | P04083 | 3.2 | 6.57/7.98 | 38.92/23.24 | 14 | 9.98E-36 | Ca2+/phospholipid binding/exocytosis | 33 |
| Annexin A1 | P04083 | 3.2 | 6.57/7.69 | 38.92/24.22 | 20 | 1.99E-43 | Ca2+/phospholipid binding/exocytosis | 37 |
| Annexin A1 | P04083 | 6.1 | 6.57/8.12 | 38.92/25.1 | 6 | 7.92E-03 | Ca2+/phospholipid binding/exocytosis | 39 |
| Calreticulin precursor | P27797 | 2.4 | 4.29/4.7 | 48.28/27.01 | 5 | 1.99E-11 | Ca2+/phospholipid binding/exocytosis | 5 |
| Cofilin-1 | P23528 | 2.1 | 8.22/6.44 | 18.72/15.89 | 12 | 6.29E-25 | Actin-related protein | 20 |
| Cofilin-1 | P23528 | 6.6 | 8.22/8.5 | 18.72/16.15 | 11 | 3.97E-20 | Actin-related protein | 28 |
| Corticotropin-lipotropin precursor | P01189 | 2.3 | 7.56/5.34 | 29.8/16.05 | 4 | 6.29 | Precursor for ACTH, MSH, beta-endorphin, and met-enkephalin | 19 |
| Ezrin | P15311 | 4.4* | 5.94/5.87 | 69.48/12.49 | 6 | 0.16 | Cytoskeletal protein | 26 |
| Fructose-bisphosphate aldolase A | P04075 | 5.2 | 8.3/8.49 | 39.85/34.54 | 14 | 1.58E-26 | Carbohydrate metabolism | 41 |
| Gelsolin precursor | P06396 | 2.9* | 5.9/5.92 | 86.04/85.18 | 26 | 3.97E-56 | Actin-related protein | 1 |
| Guanine nucleotide-binding protein subunit beta 2-like 1 | P63244 | 2.8 | 7.6/7.96 | 35.51/24.55 | 6 | 5.00E-06 | Cell signaling | 38 |
| Heat shock protein HSP 90-alpha | P07900 | 2.2 | 4.94/6 | 85.01/16.56 | 7 | 1.58E-14 | Molecular chaperone | 18 |
| Hemoglobin subunit beta | P68871 | 3.9 | 6.75/7.5 | 16.1/9.33 | 4 | 1.26E-07 | Oxygen transport | 31 |
| Histone-lysine N-methyltransferase SETDB1 | Q15047 | 5.0 | 5.74/5.7 | 145.12/18.12 | 15 | 0.20 | Regulation of gene expression | 15 |
| Keratin, type I cytoskeletal 10 | P13645 | 6.6 | 5.13/5.96 | 59.7/5 | 8 | 0.01 | Intermediate filaments | 27 |
| Keratin, type I cytoskeletal 10 | P13645 | 3.3 | 5.13/4.66 | 59.7/6.57 | 20 | 5.00E-28 | Intermediate filaments | 23 |
| Keratin, type I cytoskeletal 9 | P35527 | 4.3 | 5.19/8.35 | 62.32/14.29 | 10 | 5.00E-07 | Intermediate filaments | 29 |
| Leukocyte elastase inhibitor | P30740 | 3.0 | 5.9/4.92 | 42.83/14.17 | 8 | 5.00E-26 | Protease inhibition | 21 |
| Macrophage migration inhibitory factor | P14174 | 6.5 | 7.74/8.57 | 12.64/9.55 | 3 | 9.98E-05 | Proinflammatory cytokine | 30 |
| Neuroepithelial cell-transforming gene 1 protein | Q7Z628 | 7.3 | 9.31/8.4 | 68.15/24.37 | 8 | 0.25 | Cell signaling | 40 |
| Neutral alpha-glucosidase AB precursor | Q14697 | 2.2* | 5.74/5.79 | 107.26/101.61 | 10 | 1.26E-08 | Glycoprotein alteration | 4 |
| Plastin-2 | P13796 | 2.4 | 5.2/4.93 | 70.82/24.33 | 9 | 1.99E-08 | Actin-related protein | 7 |
| Protein disulfide-isomerase A3 precursor | P30101 | 2.1* | 5.98/5.88 | 57.15/65.95 | 19 | 9.98E-42 | Protein rearrangement/alteration | 2 |
| Protein disulfide-isomerase A3 precursor | P30101 | 2.0* | 5.98/5.55 | 57.15/70.5 | 15 | 3.15E-14 | Protein rearrangement/alteration | 3 |
| Protein S100-A9 | P06702 | 2.4 | 5.71/5.16 | 13.29/5.22 | 4 | 6.29E-03 | Ca2+ binding | 24 |
| Protein S100-A9 | P06702 | 4.7 | 5.71/5.41 | 13.29/8.96 | 5 | 9.98E-06 | Ca2+ binding | 25 |
| Synaptic vesicle membrane protein VAT-1 homolog | Q99536 | 3.9* | 5.88/7.98 | 42.12/20.97 | 4 | 9.98E-03 | Unknown function | 34 |
| Transketolase | P29401 | 5.2 | 7.58/7.34 | 68.52/73.91 | 17 | 9.98E-20 | Carbohydrate alteration | 43 |
| Vimentin | P08670 | 4.7 | 5.06/7.53 | 53.68/21.54 | 23 | 1.99E-35 | Mesenchymal intermediate filament | 32 |
| Vimentin | P08670 | 2.1 | 5.06/6.02 | 53.68/21.66 | 27 | 6.29E-29 | Mesenchymal intermediate filament | 16 |