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. 2011 Nov;10(11):1455–1464. doi: 10.1128/EC.05207-11

Table 2.

Primers used in this study

Primer Sequence (5′–3′) Description or functiona
CX5 GTAAAACGACGGCCAG M13F
CX6 CAGGAAACAGCTATGAC M13R
CX44 GAAATGTCATCGCCTGTGTGCCAA FBP1 qRT-PCR
CX45 TGTCAAACTTGATCCTGCGGAGCA FBP1 qRT-PCR
CX374 ATCGCCTCTGAAAACACTCTC HOG1 qRT-PCR
CX375 ACTCCAATCAAACACCTCGG HOG1 qRT-PCR
CX411 CTAGCCCGTTACCTCAATCTC CCK1 qRT-PCR
CX412 TTTCCCTTGTTCTCCTGGTG CCK1 qRT-PCR
CX420 GTATCCTAACGCATCTCCCTTG MPK1 qRT-PCR
CX421 GGAAACTTCTGACCTCTTCGAG MPK1 qRT-PCR
CX49 TGA GAA GGA CCC TGC CAA CA GAPDH qRT-PCR
CX50 ACT CCG GCT TGT AGG CAT CAA GAPDH qRT-PCR
CX322 TCCACCTCCCAAGTCGTCAATG CCK1 KO left
CX323 CTGGCCGTCGTTTTACTGAGTGTGGGCGATGCTAGTG CCK1 KO left
CX324 GTCATAGCTGTTTCCTGGCCATCTCTGGTACTGATGCTG CCK1 KO right
CX325 GCAAAAGAGCTTGCAGGTACAG CCK1 KO right
CX326 CTTCCAGCTCGAGCAACATCGT CCK1 KO negative
CX327 ACTCACGTCTTTGGGTCTCTGT CCK1 KO negative
CX328 CTTCATCTTGCCAAAGGGTCTC CCK1 KO positive
CX336 GGTGTAGTAGTGCTTCGTTGTG CCK1 complementation
CX337 CTGGCCGTCGTTTTAC CAACCGTAACTTCCACTCATGC CCK1 complementation
CX426 GGGGATCCGTCGACCTGCAGCCATGGCTACCACACACGTCATC Yeast expression
CX427 CGGAATTAGCTTGGCTGCAGCTATCGGAAGCAGCCGCAAG Yeast expression
MPK1-A GATCATGGCGATAGGGATAC mpk1 mutant screen
MPK1-B CTCTCATCAAGTCATCCTCC mpk1 mutant screen
CNACT7 TCCTCTCCTCCGACAACC mpk1 mutant screen
JH8994 TGTGGATGCTGGCGGAGGATA Mutant screen
a

KO, knockout.