Table 2.
Primer | Sequence (5′–3′) | Description or functiona |
---|---|---|
CX5 | GTAAAACGACGGCCAG | M13F |
CX6 | CAGGAAACAGCTATGAC | M13R |
CX44 | GAAATGTCATCGCCTGTGTGCCAA | FBP1 qRT-PCR |
CX45 | TGTCAAACTTGATCCTGCGGAGCA | FBP1 qRT-PCR |
CX374 | ATCGCCTCTGAAAACACTCTC | HOG1 qRT-PCR |
CX375 | ACTCCAATCAAACACCTCGG | HOG1 qRT-PCR |
CX411 | CTAGCCCGTTACCTCAATCTC | CCK1 qRT-PCR |
CX412 | TTTCCCTTGTTCTCCTGGTG | CCK1 qRT-PCR |
CX420 | GTATCCTAACGCATCTCCCTTG | MPK1 qRT-PCR |
CX421 | GGAAACTTCTGACCTCTTCGAG | MPK1 qRT-PCR |
CX49 | TGA GAA GGA CCC TGC CAA CA | GAPDH qRT-PCR |
CX50 | ACT CCG GCT TGT AGG CAT CAA | GAPDH qRT-PCR |
CX322 | TCCACCTCCCAAGTCGTCAATG | CCK1 KO left |
CX323 | CTGGCCGTCGTTTTACTGAGTGTGGGCGATGCTAGTG | CCK1 KO left |
CX324 | GTCATAGCTGTTTCCTGGCCATCTCTGGTACTGATGCTG | CCK1 KO right |
CX325 | GCAAAAGAGCTTGCAGGTACAG | CCK1 KO right |
CX326 | CTTCCAGCTCGAGCAACATCGT | CCK1 KO negative |
CX327 | ACTCACGTCTTTGGGTCTCTGT | CCK1 KO negative |
CX328 | CTTCATCTTGCCAAAGGGTCTC | CCK1 KO positive |
CX336 | GGTGTAGTAGTGCTTCGTTGTG | CCK1 complementation |
CX337 | CTGGCCGTCGTTTTAC CAACCGTAACTTCCACTCATGC | CCK1 complementation |
CX426 | GGGGATCCGTCGACCTGCAGCCATGGCTACCACACACGTCATC | Yeast expression |
CX427 | CGGAATTAGCTTGGCTGCAGCTATCGGAAGCAGCCGCAAG | Yeast expression |
MPK1-A | GATCATGGCGATAGGGATAC | mpk1 mutant screen |
MPK1-B | CTCTCATCAAGTCATCCTCC | mpk1 mutant screen |
CNACT7 | TCCTCTCCTCCGACAACC | mpk1 mutant screen |
JH8994 | TGTGGATGCTGGCGGAGGATA | Mutant screen |
KO, knockout.