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FLO11 expression is greatly diminished in class E-1 mutants known to affect Rim101p processing, but class E-2 mutants like vps27Δ and vps4Δ mutants do not show a decrease in FLO11 expression. Fold change in FLO11 expression was measured by rtRT-PCR, and ACT1 was used as a reference gene. WT, wild type; 27, vps27Δ; 28, vps28; 25, vps25Δ; 20, vps20Δ; 4, vps4Δ. *, P < 0.05 compared to wild type.
