Fig. 3.

The RIM101-531 allele suppresses invasive growth and FLO11 expression defects in the vps25Δ and rim13Δ mutants. (A) Strains carrying the RIM101-531 allele were subjected to the invasive growth assay. Mutants with a capital R are double mutants carrying the named mutation and the RIM101-531 allele. (B) Fold change in FLO11 expression was measured by rtRT-PCR, and ACT1 was used as a reference gene. WT, wild type; 25, vps25Δ; 25R, vps25Δ RIM101-531; 27, vps27Δ; 27R, vps27Δ RIM101-531; 13, rim13Δ; 13R, rim13Δ RIM101-531. *, P < 0.05 compared to wild type.