Fig. 6.

mCherry-Cna1 colocalizes with the P-body marker GFP-Dcp1 during exposure to a variety of stresses. Cells expressing mCherry-Cna1 and GFP-Dcp1 were initially grown at 24°C in complete synthetic medium. Subsequently, the medium was replaced with medium supplemented with KCl, LiCl, or sorbitol as indicated and cells were incubated at 24°C for 10 min prior to microscopic analysis. Arrows in the merged images indicate cytoplasmic puncta where mCherry-Cna1 and GFP-Dcp1 colocalize. The scale bars represent 5 μm.