Table 1.

Adaptive mutants isolated from both fluconazole-exposed (FLU) and control populations

Adaptive event	Strain	Colora	FLU (μg/ml)b	No. of generationsc	No drug		FLU		MIC (μg/ml)	Ploidy
					Relative fitnessd/generation	P valued	Relative fitnessd/generation	P valued
Parental	KKCa-14	RFP							0.125	2N
Parental	KKCa-17	GFP							0.125	2N
Parental	KKCa-20	YFP							0.125	2N
1	CP-1-M1	RFP	0	64	0.16 ± 0.1	0.05	NAe	NA		∼2N
2	CP-1-M2	YFP	0	70	0.05 ± 0.01	0.00	NA	NA		∼2N
3	CP-1-M3	GFP	0	106	0.15 ± 0.02	0.00	NA	NA		∼2N
4	CP-1-M4	RFP	0	158	0.24 ± 0.14	0.02	NA	NA		∼2N
5	CP-2-M1	GFP	0	76	0.3 ± 0.02	0.00	NA	NA		∼2N
6	CP-2-M2	YFP	0	125	0.11 ± 0.09	0.09	NA	NA		∼4N
7	CP-2-M3	RFP	0	172	0.09 ± 0.11	0.12	NA	NA		∼2N
8	DP-1-M1	RFP	0.25	58	0.02 ± 0.1	0.74	0.58 ± 0.13	0.01	0.38	∼2N
9	DP-1-M2	YFP	1	76	0.03 ± 0.1	0.53	0.5 ± 0.05	0.00	0.38	∼2N
10	DP-1-M3	RFP	1	93	0.02 ± 0.01	0.00	0.27 ± 0.06	0.01	1	∼2N
11	DP-1-M4	YFP	4	125	0.04 ± 0.02	0.02	0.03 ± 0.25	0.88	1	∼2N
12	DP-1-M5	GFP	4	134	−0.21 ± 0.02	0.00	0.55 ± 0.23	0.05	1.5	∼2N
13	DP-1-M6	RFP	4	146	0.09 ± 0.03	0.03	−0.17 ± 0.08	0.02	2	∼3N
14	DP-1-M7	YFP	4	172	0.13 ± 0.01	0.00	0.35 ± 0.16	0.06	3	∼2N
15	DP-2-M1	RFP	0.25	31	0 ± 0.07	0.95	0.42 ± 0.08	0.00	0.75	∼2N
16	DP-2-M2	GFP	0.25	47	0.11 ± 0.08	0.08	0.12 ± 0.11	0.20	0.5	∼2N
17	DP-2-M3	RFP	0.25	52	0.16 ± 0.1	0.11	0.16 ± 0.15	0.13	1	∼2N
18	DP-2-M4	GFP	0.25	64	0.14 ± 0.02	0.00	−0.32 ± 0.03	0.00	0.19	∼2N
19	DP-2-M5	YFP	1	76	−0.15 ± 0.04	0.02	0.68 ± 0.34	0.03	0.5	∼2N
20	DP-2-M6	RFP	1	106	0.06 ± 0.03	0.04	0.31 ± 0.20	0.12	2	∼2N
21	DP-2-M7	GFP	1	112	0.02 ± 0	0.01	−0.26 ± 0.15	0.03	0.5	∼2N
22	DP-2-M8	RFP	4	125	−0.02 ± 0.01	0.01	0.49 ± 0.15	0.03	1	∼2N
23	DP-2-M9	GFP	4	153	0.1 ± 0.02	0.01	0.4 ± 0.06	0.00	0.25	∼2N
24	DP-2-M10	RFP	4	172	−0.11 ± 0.04	0.04	0.29 ± 0.04	0.00	8	∼2N

^aFluorescent marker.

^bFluconazole concentration in the feed.

^cGeneration from which the strain was isolated.

^dRelative to previous adaptive mutant.

^eNA, not applicable.