Table 3.
Chemical sensitivity summary
| Deleted gene | Sensitivitya |
|||||||
|---|---|---|---|---|---|---|---|---|
| Cytochalasin A | Benomyl | FK506 | Sorbitol | Sodium chloride | Fludioxonil | tert-Butyl hydroperoxide | Menadione | |
| stk-10 | S | |||||||
| stk-23 | S | |||||||
| camk-4 | R | |||||||
| stk-16 | S | S | S | |||||
| prk-10 | R | S | ||||||
| mus-58 | S | |||||||
| prk-3 | S | |||||||
| div-4 | S | |||||||
| stk-47 | S | |||||||
| cka | S | |||||||
| stk-46 | S | |||||||
| gsk-3 | R | |||||||
| ime-2 | R | |||||||
| stk-54 | R | |||||||
| stk-22 | R | R | ||||||
| ptk-2 | S | |||||||
| stk-30 | S | |||||||
| stk-5 | S | |||||||
| stk-38 | S | S | S | |||||
| stk-9 | S | S | S | |||||
| nim-1 | R | |||||||
| stk-17/fi | S | |||||||
| apg-1 | S | S | S | S | S | |||
| stk-29 | S | |||||||
| stk-4 | S | S | ||||||
Mutants were classified as sensitive (S) or resistant (R) relative to the wild type based on the percentage of growth in the presence of the chemical compared to growth in the absence of the chemical, as follows: percent growth = (colony radius with chemical)/(colony radius without chemical) × 100. See Materials and Methods for details. For all compounds, sensitivity was scored directly from the average growth rate, except for menadione, for which sensitivity was scored after the average growth rates were binned (see Table S1 in the supplemental material). Corroboration of findings was based on the version 5 annotation of the Broad Institute's Neurospora crassa database (www.broadinstitute.org/annotation/genome/neurospora/MultiHome.html).