Fig. 2.
ras2 mutation resulted in increased switching rates. (A) Schematic drawing of the genetic selection used for isolating strains with increased switching rates. The starting strain (mata2Δ::NAT hmlα1::kanMX), which was G418s and sterile, was mutagenized, and revertant strains were isolated that were G418r (matα1Δ::kanMX) and fertile (MATa). (B) DNA blot analysis of BamHI-digested DNA from SAY129 (MATα), SAY130 (MATa), SAY990 (mata2Δ::NAT), and SAY1069 (isolated from the selection described for panel A, ras2::pLEU2) hybridized with a MAT-specific probe. The MATα, MATa, mata2Δ::NAT, and matα1Δ::kanMX bands are indicated.