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Crossing point (Cq) values for Aspergillus PCR when testing DNA extracted from serum spiked with various fungal burdens as determined by linear regression. The linear equation for the mean Cq values is y = −3.76 log(x) + 42.67, where log(x) = log10 genomes/ml. The 95% CI for the slope was −3.49 to −4.02.
